Training programmes at TANUVAS

TAMIL NADU VETERINARY AND ANIMAL SCIENCES UNIVERSITY

DEPARTMENT OF ANIMAL BIOTECHNOLOGY

FACULTY OF BASIC SCIENCES

MADRAS VETERINARY COLLEGE, CHENNAI - 600 007

HUMAN RESOURCE DEVELOPMENT UNIT

• The Department of Animal Biotechnology with its state of the art facilities and well-qualified faculty by offering training programmes to interested researchers and students, contribute to the development of quality human resource in this frontier area

• This could reduce the shortage to quality manpower and thus facilitate our nation to harness this frontier area of science for human and animal welfare

The Human Resource Development (HRD) unit of this Department offers the following levels of training programmes:

LEVEL I TRAINING – SHORT TERM TRAINING FOR 5 DAYS DURATION

The training would be on any of the following areas:

AREAS OF TRAINING

Tentative topics to be covered under the training

 PCR and related techniques

DNA isolation, PCR, RT-PCR, Nested PCR, Multiplex PCR and RAPD

 Gene cloning and expression

Preparation of vector and insert DNA, ligation, transformation, colony PCR, Induction and expression of recombinant protein, SDS-PAGE

 Real time PCR and sequencing techniques

General overview of real time PCR, Primer design, real time PCR by SYBR / TaqMan chemistries, Dissociation curve analysis, PCR product purification, cycle sequencing, Dye Terminator clean up, Sequencing of samples, sequence analysis, BLAST analysis

 Animal Cell culture

Media preparation, Maintenance of cell lines, Primary cell culture, Cytotoxic assay, Cryopreservation of cell lines and Karyotyping

 Isolation and characterization of adult/embryonic stem cells

Preparation of Media and reagents for stem cell isolation, Magnetic activated cell separation using cell surface marker, alkaline phosphatase activity and fluorescence / flow cytometry of cultured cells

 Diagnostic techniques

Rabies virus antigen detection by fluorescence antibody test, Rapid fluorescence foci inhibition test, SDS-PAGE, Western blot and latex agglutination test

 Flow cytometry

Demonstration and handling of flow cytometer, CellQuest software, isolation of chicken mononuclear cells, staining of chicken mononuclear cells for CD4 and CD8 and cell cycle analysis

 Protein purification

Ammonium sulfate precipitation , affinity column purification, protein separation by SDS-PAGE, Western blot, ELISA

 Application of bioinformatics tools

Primer design for PCR, dendrogram, multiple sequence alignment, sequence assembly, EST analysis and Gene annotation (Blast2go), Phylogenetic analysis-MEGA (using Bestfit method), Lasergene software and Protein modeling

 Typing of bacteria

Bacterial isolation and characterization, DNA extraction, 16s rDNA PCR, RAPD, sequencing and typing of bacteria based on sequence results

 In vitro maturation and fertilization

Harvesting and grading of oocytes, separation and analysis of sperm, assessment of fertilization, preparation of oviduct epithelial cell culture and cleavage studies

 Cryopreservation of oocytes and embryo

Principles of cryopreservation, demonstration of programmable cell freezer, preparation of cryopreservation media, slow freezing and vitrification procedure, post thaw assessment of oocytes/ embryos

 Micromanipulation technique

Preparation of holding and injection pipette, embryo sexing by blastomere aspiration. Demonstrations on somatic cell nuclear transfer, transgenic animal production and siRNA injection

 Ultrasonography studies of reproductive parameters in farm animals

Principles of ultrasonographic imaging, demonstration of ultrasonogram equipments, assessing the follicular and luteal structures under physiological and pathological condition, pregnancy diagnosis, ovum pick-up methods

• This level of training involves 80% of hands-on training component and ~20% theoretical aspects

• The training fees is Rs. 8000/- per individual

• A minimum of 2 and a maximum of 5 participants would be permitted at a time

• Students and researchers having sufficient basic knowledge on life sciences / biotechnological techniques can apply any time of the year

• An application in plain paper indicating your address, qualifications and other academic details along with a bonafide certificate from your institution may be submitted anytime of the year

• The time for the training may be fixed in consultation with the concerned faculty involved in offering training in that specified area

LEVEL II – LONG TERM OVERVIEW TRAINING FOR 28 DAYS DURATION

• This training is meant to give an overview of various facets of Biotechnology namely, Molecular biology, Immuno technology, Cell culture and Hybridoma technology and Embryo and related biotechnologies including stem cells.

• This involves ~80% of theoretical aspects and demonstration s with ~20% of ‘hands-on’ training.

• The training fees is Rs. 10,000/- per individual

• A maximum of 32 participants would be permitted at a time

• Both undergraduate and post graduate students of any science discipline who wants to get on overall insight into biotechnology can apply

• An application in plain paper indicating your address, qualifications and other academic details (including photocopy of the mark sheet of the last university examination passed) along with a bonafide certificate from your institution may be submitted during January - March of each year

• Selection is based on the marks / CGPA obtained in the last University Examination passed

• The training is usually held in May-June every year

TOPICS TO BE COVERED DURING THE TRAINING

Animal Cell culture

Primary cell culture, Sub culture, Cryopreservation, Cytotoxicity assay, Maintenance of cell lines, Cell counting

Molecular Biology

Isolation of DNA/RNA from blood and bacteria, PCR, RE digestion, RT-PCR, Real time PCR and Sequencing

Immuno technology

SDS-PAGE, Western blotting, ELISA methods, Flow cytometry, Fluorescence antibody technique and monoclonal antibody production, isotyping.

Embryo Biotechnology

Oocyte collection and grading, In vitro maturation, In vitro fertilization, Cryopreservation of oocyte and embryos, embryo sexing, gene expression in embryos, stem cells isolation methods

LEVEL III – PROJECT WORK OR IN-PLANT TRAINING FOR 3 MONTHS DURATION

• This programme is intended for students doing their final year research projects / in-plant training with 100% ‘hands-on’ work experience.

• The training fees is Rs. 20,000/- per individual.

• A maximum of 30 participants would be permitted at a time.

• The Project work may be chosen in the broad areas of Molecular Biology, Animal Cell Culture, Immuno technology, Animal Genomics and Embryo biotechnology including stem cells in consultation with the concerned faculty.

• An application in plain paper in the prescribed format may be submitted during June-August of each year.

• Selection would be based on the academic performance of the candidate along with their statement of purpose of the Project Work to be undertaken

• The training is usually held in November- February of each academic year.

All payments should be made only after confirmation of selection of the candidate

Payments should be made in the form of Demand Draft to the address below payable at Chennai

CONTACT:The Professor and Head

Department of Animal Biotechnology

Madras Veterinary College, Chennai – 600 007

Phone: 044-25304000 Ext. 2032, 25369301

Email – hodabtmvc@tanuvas.org.in

FOR DETAILS, PLEASE REFER http://www.tanuvas.tn.nic.in/nea/docs/ABT-hrd.pdf