5th practical summer training on chromatography, molecular biology & bioinformatics (Pcmb)
Organized by
School of Bio Sciences and Technology (SBST)
VIT University, Vellore – 632 014, Tamil Nadu, India
VIT – A place to learn; A chance to grow
ABOUT VIT UNIVERSITY
Founded in 1984 as Vellore Engineering College, the institute was declared a University in recognition of its academic excellence by the Ministry of Human Resources Development, Government of India in 2001. The University has since grown by leaps and bounds – establishing excellent infrastructure replete with state-of-the-art laboratories, digital library, smart class rooms, hostels, gyms, swimming pools, auditoria and stadium spread over an impeccably clean and green 250 acre campus - on the way to fulfill the vision of the founder and Chancellor Dr. G. Viswanathan to make it truly World class. VIT today comprises of nine constituent Schools and interdisciplinary Centers offering undergraduate, graduate, post graduate and research programs upto PhD level.
VIT is the first educational institution of India to get ISO 9002 Certificate by the DNV of The Netherlands. It is again the first Institute in India to get accreditation from IEE (UK). Further it has also been accredited by NBA (AICTE) and NAAC (UGC). VIT has signed MoUs with Institutions within India and from countries like Australia, Canada, France, Germany, Italy, UK and USA in various disciplines towards exchange of faculty and students, joint programs involving courses as well as research and improvement of laboratory and library facilities.
ABOUT SBST
School of Bio Sciences and Technology is offering B. Tech, M. Tech., M. Sc., and Ph. D. programmes in Biotechnology and many other allied areas. SBST has adequate infrastructure for carrying out academic, research and developmental activities in different areas of Biotechnology. Major areas of research include Molecular Genetics, Plant Biotechnology, Microbiology, Cancer Biology, Bioinformatics and Biomedical Engineering.
As a part of SBST extension activity, both for students and faculty members, PCMB is being organized every summer. For the fourth consecutive year, we are organizing the 5th practical training during the summer 2012.
SCOPE OF TRAINING
This course aims to provide exposure to Chromatography, Bioinformatics and Molecular Biology techniques. Experts from academic and industry will be involved as resource persons. The participants will be interacting with experts and will get acquainted with practical aspects of various techniques along with the underlying principles. Experiments will be conducted in small groups under the expert supervision where individual participants will follow experimental protocol designed in different areas. Practical demonstrations and lectures related to different experiments are part of the workshop.
TECHNIQUES
Cloning of DNA in bacterial expression vectors: PCR amplification of gene → Gel purification of DNA → Restriction digestion → Ligation → Bacteria Transformation
Expression and purification of proteins expressed in bacteria : IPTG induction of gene expression in BL 21 bacteria → Purification of GST tag protein from bacteria → Analysis of purified protein by SDS PAGE
Chromatography : Gel permeation Chromatography, Ion exchange Chromatography & HPLC
Techniques in Bioinformatics and Biophysics
Enriched mRNA preparation – cDNA synthesis – Semi-quantitative RT-PCR
Real time PCR
Primer design for smart cloning in vectors, primer design for gene expression studies, primer editing, probe design.
ELIGIBILITY AND SELECTION CRITERIA
Students of M. Sc / M.Tech / B.Tech in Biochemistry, Microbiology, Life Sciences, Biotechnology or Research Scholars / Faculty members / industrialists belonging to above field are eligible to apply. Only 40 candidates will be selected by first come first serve basis. The selected candidates will be informed by email on or before May 04, 2012.
REGISTRATION FEES (REGISTRATION FEES INCLUDES REGISTRATION KIT AND WORKING LUNCH)
• Rs.6,000 for students and research scholars
• Rs.7,500 for faculty members and industrial persons
Accommodation (Will be arranged on request only in VIT Hostel)
Hostel - Rs.150 per day
For further details about hotels in Vellore visit http://www.vit.ac.in/guesthouse/hotels.asp
REGISTRATION
Send the registration form along with the prescribed fee as a DD (draft in favour of VIT University, payable at Vellore) to the Organizing Secretary on or before May 4, 2012.
ADDRESS FOR COMMUNICATION
Dr. K. M. Gothandam
Organizing Secretary, PCMB
SBST, VIT University
Vellore - 632 014, Tamil Nadu.
Ph.: 0416 2202616; 9489337967
E-mail: pcmbsbst@gmail.com
Website : www.vit.ac.in/pcmb
School of Bio Sciences and Technology
FOR DETAILS, PLEASE REFER http://www.vit.ac.in/events2012/Brochure_PCMB_2012.pdf
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