A Training Course on “Molecular Diagnostics & Bioinformatics Tools”
From 8th to 28th November, 2011
COURSE COORDINATOR: Dr. M.L. Sangwan
COURSE FACULTY: Drs. Trilok Nanda, Minakshi, & Aman Kumar
ORGANISED BY
Department of Animal Biotechnology
College of Veterinary Sciences
Lala Lajpat Rai University of Vety. & Animal Sciences
Hisar 125 004, Haryana
DURATION: 8th November to 28th November, 2011
COURSE FEES: Participants are requested to pay a sum of Rs. 5000 (Rs five thousand) as registration fee. The registration fee shall be deposited in cash at the time of registration on November 8, 2011.
LABORATORY AND COMPUTING FACILITIES: The laboratories are well equipped with modern equipments and other lab wares.
ACCOMMODATION: Arrangements for the stay of the participants during the training program will be made in faculty house of the university on the payment basis.
NUMBER OF PARTICIPANTS: The maximum number of participants shall not exceed 20.
PARTICIPANTS AND ELIGIBILITY: Participants are invited from ICAR Institutes/SAU/Basic Science Institutes/ Sate Governments/Private Organizations. They should be graduates having interest in the training.
OBJECTIVE:
The science of diagnostics gets revolutionized with the development of Molecular biology tools as it provides high level of sensitivity and specificity to the diagnostics. These tools can detect various pathogenic organisms like bacteria, viruses, etc. through the presence of DNA, RNA or protein sequences in clinical samples. Nowadays commercial kits are available to detect pathogens directly from clinical
samples and make it possible to obtain results in just a few minutes or hour, but most of these kits are exogenous in origin and thus very much expensive. Therefore, it is important that highly specific diagnostic tests should be developed indigenously based on the Indian strains of pathogens. Hence, the present practical training course is designed to provide hands on training on basic molecular biology techniques used for diagnosis of pathogens in blood, semen, clinical tissues, faecal samples and characterization of pathogens and breeds of animals. For better understanding of data of molecular biology, it is essential to have the basic knowledge of bioinformatic tools; keeping these points in view this training programme will also include hands on training on bioinformatics tools.
COURSE CONTENTS:
Ø Preparation of reagents, buffer and media.
Ø Extraction and purification of nucleic acid of viral agents from various sources like blood, semen, clinical tissues, faecal samples, etc.
Ø Electrophoretic separation of nucleic acid and protein.
Ø RNA-Polyacrylamide gel electrophoresis for segmented viruses.
Ø Template preparation for Polymerase chain reaction (PCR)
Ø Amplification of gene of interest using conventional PCR
Ø Synthesis of cDNA and RT-PCR based amplification of viral genes.
Ø DNA markers like RAPD,RFLP etc.
Ø Molecular cloning of genes in suitable vectors
Ø Sequence alignment tools e.g. BLAST
Ø Designing of PCR Primer
Ø Analysis of nucleotide sequences.
Ø Phylogenetic analysis of nucleic acid sequence data.
HOW TO APPLY: The application for participation may be sent in prescribed format, duly forwarded by head of the institution. It should reach to the Course Director latest by November 1st, 2011 by post, fax or by email .TA & DA of the participants will be borne by participants/sponsoring institutions/ organizations etc. The participants will also have to pay for their boarding and lodging charges during the training program. The organizers of the course will not bear any expenses on account of the participants. The participants will be informed about their selection by November 4th, 2011.
All correspondence may please be addressed to:
Dr. M.L. Sangwan, Course Coordinator
Department of Animal Biotechnology, COVS
LLRUVAS, Hisar
Phone no. 01662- 289132 (office)
01662- 244384 (Res), Cell: 09896588704
Fax 01662-234613, 234952
Email: abt@hau.ernet.in, mlsangwan@hau.ernet.in
Photocopy of the application form can be used
Information can also see on website (www.hau.ernet.in)
FOR DETAILS, PLEASE REFER http://www.hau.ernet.in/abtt.pdf
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