HAND-ON TRAINING ON PLANT TISSUE CULTURE, MOLECULAR BIOLOGY AND IMMUNOLOGICAL TECHNIQUES FOR STRENGTHENING OF FOUNDATION IN BIOTECHNOLOGY

Silver Jubilee Year (1988 –2013)Pantnagar Biotechnology Program

G.B. Pant University of Agriculture & Technology,

College of Basic Science & Humanities,

Department of Molecular Biology & Genetic Engineering

INVITES APPLICATION FOR SUMMER TRAINING (2012-13)

ON

“HAND-ON TRAINING ON PLANT TISSUE CULTURE, MOLECULAR BIOLOGY AND IMMUNOLOGICAL TECHNIQUES FOR STRENGTHENING OF FOUNDATION IN BIOTECHNOLOGY”

GBPantUniversityofAgricultureandTechnologyisoneoftheprestigiousuniversitiesinthefieldofagricultureandtechnology.TheuniversityhasgreatstrengthinMicrobiology,Biochemistry,MolecularBiology,Vaccines,Biocontrol,Biofertiizer,PostHarvestandProcessEngineering.Theuniversityhastheremarkablehistoryofproducingengineeringandagriculturegraduatesandpostgraduateswhoareoccupyingpremierpositionngovernmentaswellasprivateorganizations.Biotechnologycoursesofferedbytheuniversityareupheldnotonlyatthenationallevelbutalsoattheinternationallevel.DepartmentofMolecularbiologyandGeneticEngineering,CollegeofBasicScienceandHumanitiesisanodaldepartmentandsuccessfullyrunningMaster’sandDoctoralprogrammesinMolecularBiology&Biotechnologysincelasttwodecades.Keepingpacewiththepresentscenarioofthebiotechindustry,theuniversityisofferingB.Techcourseinbiotechnology(purelyselffinancing)toenablethestudentstoexploregreatercareerprospectsandemploymentopportunities.ThebiotechnologyprogrammeofthisuniversityhasbeenadjudgedasoneoftheTop-Tenschoolinthecountry.

MODULE I PLANTTISSUECULTURE

·         HowtomaintaingoodlaboratoryPractices

·         Biochemicalcalculations

·         LaboratoryInstruments,principleandtheiruses

·         PreparationofMSmedia

·         Callusinductionfromdifferentexplants

·         Regenerationofcallus(Shootandrootdevelopment)

·         Micropropagation/multipleshootinduction

·         Immobilizationofcells

·         Cryopreservationsomaticcellsandmeristamatictissues

MODULEII MOLECULAR BIOLOGY AND BIOINFORMATICS

·         IsolationofgenomicDNAfromdifferentsources

·         IsolationofplasmidDNA

·         DNAQuantification

·         AgroseGelElectrophoresis

·         purificationofDNAfragmentsfromagrosegel

·         Restrictiondigestion

·         DataMiningGENBANK

·         BlastingSequence

·         Primerdesigning

·         PreparationofvectorDNA

·         PreparationofInsertDNA

·         Purificationofvectorandinsertfromagrosegel

·         Ligation

·         PreparationofCompetentcells

·         Transformation

·         Screeningofclonesusingrestrictiondigestion

·         VariantsofPCRandAmplificationofgene

·         RT-PCR

MODULE III PROTEINPURIFICATIONTECHNIQUES

·         Bufferpreparation

·         Extractionofproteinfromdifferentsources

·         ProteinConcentrationTechniques

·         Dyalysis

·         PricipitationTechniques

·         ColumnChromatography

·         Quantificationofprotein

·         SeparationofproteinbySDS-PAGE

·         UseofRadioisotopetechniques

·         MODULEIV IMMUNOLOGY

·         ImmunizationofRabbit

·         Determinationofantibodytitre

·         Latexagglutinationtest

·         RapidimmunologydiagnosticprocedureforSTDs

·         Radialimmunodiffusiontest

·         Quantitativeprecipitinassay

·         IndirectELISA

·         WesternBlotting

UNIQUE FEATURES OF TRAINING

·         A strong emphasis on hands on laboratory session.

·         Faculty are more experienced in their respective area.

·         Limited enrollments with an individualized learning environment.

·         Curriculum and experienced speaker are carefully selected to emphasize on practical approaches.

ELIGIBILITY: Enrollment of the candidates in B.Sc. / B. Tech. (Biotech) / M. Sc. / M. Tech. (Biotech) programme

BENCH FEE: Rs. 10,000.00/ for DBT sponsored Institute and Rs. 15,000.00 for non DBT sponsored institute

DURATION: Four to six weeks (June 10th-July 15th, 2012)

HOW To APPLY: The candidate should send their Bio-data along with recommendation letter from Head of  the Institute/Principal/ Dean of the College in the office of Head MBGE,C.B.S.H, G.B.Pant University of Agriculture and Technology Pantnagar, District U.S.Nagar–263145(Uttrakhand)latest by May15th 2012.The Incomplete applications or received after due date will not be considered.

FOR FURTHER CLARIFICATION ,YOU MAY CONTACT

Dr.AnilKumar(Professor&Head)

DepartmentofMolecularBiology&GeneticEngineering

Coordinator,PantnagarBiotechProgramme

Ph.05944-233898(O),9411195450(M)

Fax.05944-233473

e-mali: ak_gupta2k@rediffmail.com 

Dr.B.R.Singh(AssttProf.)DepartmentofMolecularBiology&GeneticEngineeringPh.9412323616e-mail: singhbr@rediffmail.com

FOR DETAILS, PLEASE REFER http://www.gbpuat.ac.in/news/June-July%20summer%20Training.pdf