2-Day (Hands on) Workshop on ENZYME ENGINEERING AND
PROCESS OPTIMIZATION PRINCIPLES by Centre for Innovative Research (CIR) and Centre
for Biotechnology (CBT) IST, JNTUH, Hyderabad
March (16th-17th, 2012)
Scope
of the work shop:
The
program emphasis is on hands on experience on various techniques involved in
Enzyme
engineering
& technology and Process optimization principles for enhancement of
industrially
important
enzymes production. It aims to enable the handling experience on various techniques
to
the students in the field of Pharmacy & Industrial Biotechnology.
Eligibility:
M. Sc (Life sciences (plant & animal), Biochemistry,
Microbiology, Biotechnology,
Bioinformatics
and Genetics) and M. Tech. (Biotechnology &Chemical Engineering), Students
of
B. Tech (Biotechnology& Chemical Engineering), Pharmacy (M.Pharm
&B.Pharm).
Programme
venue – CIR, 4th floor, IST,
JNTUH, Kukatpally, Hyderabad.
Registration
Fee: Rs 1200/- (DD drawn in favour of Director, IST, CIR account, IST,
JNTUH.
Payable at Hyderabad)
No.
of registrations are limited to 15.
Last
date for registration-15/02/2012 (spot registrations are also available)
Details
also available on JNTUH website www.jntuh.ac.in
Topics to be covered
DAY1
Lecture:
Introduction to Enzyme Engineering and its Importance on
Biotechnology Research,
RSM,
Process optimization for Enzyme production in a fermenter
Practical
Sessions:
Enzyme extraction by centrifugation, filtration
Enzymatic assay by Spectrophotometer, HPLC, Titrimetry and ELISA.
Statistical optimization of enzyme, Process optimization for
Enzyme production in a
fermenter
Concentration of Enzyme by Lyophilization and Precipitation,
Dialysis.
DAY
2
Lecture:
Importance of Enzyme kinetics, Enzymes as drug targets (Drug
inhibitors)
and
different purification methods for Enzymes, Immobilization Techniques
Practical
Sessions:
Enzyme Kinetics-Km, Vmax-MM and LB plots, Enzymes as
drug targets (Drug
inhibitors)
Determination of mass transfer coefficient using sodium sulphite
oxidation method and
dynamic
gassing method in 10 L in situ fermenter
Purification of Enzyme by FPLC- Ion exchange chromatography
SDS-PAGE
Western blotting/Isoelectric focusing
Enzyme immobilization by different
matrices
FOR
DETAILS, PLEASE REFER
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