CU
BD CoE for Nanobiotechnology
Centre
for Research in Nanoscience and Nanotechnology
University
of Calcutta, JD-2, Sector-III, Kolkata-98
Training
Courses for the Year 2012
|
Date
|
Dec
26-28
|
Nov
21-23
|
Sep
19-21
|
July
25-27
|
June
26-28
|
May
23-25
|
April
25-27
|
March
21-23
|
|
Course
|
Flow*
|
Sort
|
Flow#
|
Nano/Apo
|
HCI
|
Flow*
|
Signaling
|
Flow
|
• BASIC COURSES:
– BASICS OF FLOW CYTOMETRY (BD FACSARIA IIITM) (COURSE CODE– FLOW): A
three day course on basic research Flow Cytometry for students or researchers
with no/ minimal hands on experience. This course covers theory of Flow
Cytometry, and its research applications. Hands on session will include routine
procedure of instrument operation, quality control, data acquisition and data
analysis using BD FACSDivaTM software. The data analysis will cover the
concepts of color compensation, cell cycle analysis and multicolor
immunophenotyping.
– MICROSCOPY AND
HIGH CONTENT IMAGING (BD PATHWAY 855TM) (COURSE CODE- HCI): A three day course on
Fluorescence/ Confocal Microscopy and High Content Image analysis for
researchers with varied level of experience. This course encompasses theory and
concepts of high content imaging and image analysis. The wet lab session will
cover High Content Imaging of live and fixed cells followed by Image data
acquisition, image analysis with BD Attovision softwareTM and image data
analysis with BD IDE software.
• ADVANCED
CYTOMETRY COURSES:
Advanced cytometry courses are
designed for researchers with more than 6 months of experience with image and flow
based cytometric techniques pertaining to the following topics
–
NANOBIOTECHNOLOGY/ APOPTOSIS (COURSE CODE- NANO/APO): This advanced module will cover
Flow cytometric Study of NP uptake by cells, study of apoptosis by flow and
image based cytometry. The wet lab will include the following methods: Annexin
V/ PI double staining, BrdU incorporation (TUNNEL) assay, Apoptosis associated
mitochondrial membrane potential study by JC1.
– CELL SORTING
(COURSE CODE- SORT):
This advanced module will cover theory of flow cytometric sorting, concept of
drop delay, different sorting modes and sort masks. The wet lab will include 2
way and 4 way sorting of beads and sorting of rare population of cells (Treg
cells), cell sorting to 96 well plate will be demonstrated.
– CELL SIGNALING (COURSE CODE- SIGNALING): This module will cover
theory of cell signaling studies by flow and image cytometry. The wet lab will
include stimulation of whole blood using different stimulators and analysis of
phosphorylation in T lymphocytes. For the study of phosphorylated proteins from
cell lysates the BD CBA method will be used.
ACCOMMODATIONS: Accommodations can be arranged
at nearby guest house/ hotels on request and payments to be settled by the
participants directly to the guest house/ hotel.
CONTACT: Sanjaya k Mallick, PhD.
Application Scientist and CoE Manager.
EMAIL: sanjaya_mallick@bd.com , Mob: +91
9836763355
Atanu Roychowdhury,
Administrative officer CRNN, Email: atanu.roychowdhury@ymail.com
FOR DETAILS, PLEASE REFER http://www.caluniv.ac.in/News%20&%20Announcement/CU%20BD%20CoE%20Training%20Calendar%202012.pdf
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